Antibody was affinity purified using an epitope specific to Gemin4 immobilized on solid support. The epitope recognized by A300-867A maps to a region between residue 1010 and the C-terminus (residue 1058) fo human Gem (nuclear organelle) Associated Protein 4 using the numbering given in entry NP_056536.1 (GeneID 50628). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
component of gems 4, gem-associated protein 4, gemin-4, HC56, HCAP1, HCC-associated protein 1, HHRF-1, NEDMCR, p97
between 1010 and C-term
2 - 8°C
Detection of human Gemin4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Gemin4 antibody BL3554 (Cat. No. A300-867A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Gemin4 was also immunoprecipitated by rabbit anti-Gemin4 antibody BL3553, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).