Antibody was affinity purified using an epitope specific to RCC1 immobilized on solid support. The epitope recognized by A300-866A maps to a region between residue 1 and 50 of human Regulator of Chromosome Condensation 1 using the numbering given in entry NP_001260.1 (GeneID 1104 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell cycle regulatory protein, CHC1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, RCC1-I, regulator of chromosome condensation, SNHG3-RCC1, SNHG3-RCC1 readthrough
between 1 and 50
2 - 8°C
Detection of human RCC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RCC1 antibody A300-866A (lot A300-866A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RCC1 antibody A300-866A (lot A300-866A-2) used for IP at 6 µg per reaction. RCC1 was also immunoprecipitated by rabbit anti-RCC1 antibody A302-604A. For blotting immunoprecipitated RCC1, A300-866A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.linelineline