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Goat anti-RCC1 Antibody, Affinity Purified

Catalogue number:
A300-866A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to RCC1 immobilized on solid support. The epitope recognized by A300-866A maps to a region between residue 1 and 50 of human Regulator of Chromosome Condensation 1 using the numbering given in entry NP_001260.1 (GeneID 1104 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

cell cycle regulatory protein, CHC1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, RCC1-I, regulator of chromosome condensation, SNHG3-RCC1, SNHG3-RCC1 readthrough

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RCC1 by western blot.

Detection of human RCC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RCC1 antibody A300-866A (lot A300-866A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RCC1 by western blot

Detection of human RCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RCC1 antibody A300-866A (lot A300-866A-2) used for IP at 6 µg per reaction. RCC1 was also immunoprecipitated by rabbit anti-RCC1 antibody A302-604A. For blotting immunoprecipitated RCC1, A300-866A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.linelineline