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High Quality Recombinant Rabbit Monoclonal Antibodies
Bromodomain-Containing (BRD) Antibodies

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Rabbit anti-RBM9 Antibody, Affinity Purified - A300-864A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RBM9 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-864A
Name:
Rabbit anti-RBM9 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RBM9 immobilized on solid support.The epitope recognized by A300-864A maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 9 using the numbering given in entry NP_055124.1 (GeneID 23543).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
dJ106I20.3, fox-1 homolog B, fox-1 homologue, FOX2, Fox-2, fxh, hexaribonucleotide-binding protein 2, HNRBP2, HRNBP2, RBM9, repressor of tamoxifen transcriptional activity, RNA binding protein fox-1 homolog 2, RNA binding protein, fox-1 homolog 2, RNA-bin
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human RBM9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody A300-864A (lot A300-864A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of mouse and rat RBM9 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody A300-864A (lot A300-864A-2) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody A300-864A (lot A300-864A-2) used for IP at 6 µg/mg lysate. RBM9 was also immunoprecipitated by a previous lot (lot A300-864A-1) of this antibody. For blotting immunoprecipitated RBM9, A300-864A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human and mouse RBM9 by immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-RBM9 (Cat. No. A300-864A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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