Antibody was affinity purified using an epitope specific to E1B-AP5 immobilized on solid support. The epitope recognized by A300-863A maps to a region between residue 800 and the C-terminus (residue 856) of human E1B-55kDa-Associated Protein 5 using the numbering given in entry NP_008971.2 (GeneID 11100). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
adenovirus early region 1B-associated protein 5, E1B 55kDa associated protein 5, E1B-55 kDa-associated protein 5, E1BAP5, E1B-AP5, heterogeneous nuclear ribonucleoprotein U-like protein 1, HNRPUL1
between 800 and C-term
2 - 8°C
Detection of human E1B-AP5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-E1B-AP5 antibody BL3583 (Cat. No. A300-863A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). E1B-AP5 was also immunoprecipitated by rabbit anti-E1B-AP5 antibody BL3581 (Cat. No. A300-862A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).