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Rabbit anti-E1B-AP5 Antibody, Affinity Purified - A300-863A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-E1B-AP5 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-863A
Name:
Rabbit anti-E1B-AP5 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to E1B-AP5 immobilized on solid support.The epitope recognized by A300-863A maps to a region between residue 800 and the C-terminus (residue 856) of human E1B-55kDa-Associated Protein 5 using the numbering given in entry NP_008971.2 (GeneID 11100).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
adenovirus early region 1B-associated protein 5, E1B 55kDa associated protein 5, E1B-55 kDa-associated protein 5, E1BAP5, E1B-AP5, heterogeneous nuclear ribonucleoprotein U-like protein 1, HNRPUL1
Species Reactivity:
Human
Immunogen:
Between 800 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human E1B-AP5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-E1B-AP5 antibody BL3583 (Cat. No. A300-863A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). E1B-AP5 was also immunoprecipitated by rabbit anti-E1B-AP5 antibody BL3581 (Cat. No. A300-862A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

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Detection of human E1B-AP5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E1B-AP5 (Cat. No. A300-863A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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