Rabbit anti-E1B-AP5 Antibody, Affinity Purified - A300-863A - 20 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to E1B-AP5 immobilized on solid support. The epitope recognized by A300-863A maps to a region between residue 800 and the C-terminus (residue 856) of human E1B-55kDa-Associated Protein 5 using the numbering given in entry NP_008971.2 (GeneID 11100). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human E1B-AP5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-E1B-AP5 antibody BL3583 (Cat. No. A300-863A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). E1B-AP5 was also immunoprecipitated by rabbit anti-E1B-AP5 antibody BL3581 (Cat. No. A300-862A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).
Detection of human E1B-AP5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E1B-AP5 (Cat. No. A300-863A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB
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