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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-JMJD2A Antibody, Affinity Purified - A300-861A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JMJD2A Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-861A
Name:
Rabbit anti-JMJD2A Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to JMJD2A immobilized on solid support.The epitope recognized by A300-861A maps to a region between residue 1010 and the C-terminus (residue 1064) of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
JHDM3A, jmjC domain-containing histone demethylation protein 3A, JMJD2, JMJD2A, jumonji C domain-containing histone demethylase 3A, jumonji domain containing 2, jumonji domain containing 2A, jumonji domain-containing protein 2A, lysine (K)-specific demeth
Species Reactivity:
Human, Mouse
Immunogen:
Between 1010 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse JMJD2A by western blot. Samples: Nuclear extract (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody A300-861A (lot A300-861A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JMJD2A antibody A300-861A (lot A300-861A-2) used for IP at 6 µg per reaction. JMJD2A was also immunoprecipitated by rabbit anti-JMJD2A antibody A300-860A. For blotting immunoprecipitated JMJD2A, A300-861A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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