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Rabbit anti-DHX38 Antibody, Affinity Purified - A300-859A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DHX38 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-859A
Name:
Rabbit anti-DHX38 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DHX38 immobilized on solid support.The epitope recognized by A300-859A maps to a region between residue 1200 and the C-terminus (residue 1227) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 38 using the numbering given in entry NP_054722.2 (GeneID 9785).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase DHX38, DDX38, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38, DEAH (Asp-Glu-Ala-His) box polypeptide 38, DEAH box protein 38, pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, PRP16, PRP16 homolog of S.cerevisiae, PRPF
Species Reactivity:
Human, Mouse
Immunogen:
Between 1200 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse DHX38 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DHX38 antibody BL3386 (Cat. No. A300-859A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody BL3385 (Cat. No. A300-858A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

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Detection of human DHX38 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 (Cat. No. A300-859A Lot1) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB

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