Antibody was affinity purified using an epitope specific to DHX38 immobilized on solid support. The epitope recognized by A300-858A maps to a region between residue 1150 and 1200 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 38 using the numbering given in entry NP_054722.2 (GeneID 9785). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DHX38, DDX38, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38, DEAH (Asp-Glu-Ala-His) box polypeptide 38, DEAH box protein 38, pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, PRP16, PRP16 homolog of S.cerevisiae, PRPF
between 1150 and 1200
2 - 8°C
Detection of human DHX38 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX38 antibody BL3385 (Cat. No. A300-858A) used for IP at 3 µg/mg lysate. DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody BL3386 (Cat. No. A300-859A), which recognizes a downstream epitope. For blotting immunoprecipitated DHX38, BL3386 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.