Antibody was affinity purified using an epitope specific to HURP/DLG7 immobilized on solid support. The epitope recognized by A300-853A maps to a region between residue 800 and the C-terminus (residue 846) of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DAP-5, Discs large homolog 7, discs large homolog associated protein 5, discs, large homolog 7, disks large-associated protein 5, disks large-associated protein DLG7, DLG7, hepatoma up-regulated protein, HURP
between 800 and C-term
2 - 8°C
Detection of human HURP/DLG7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HURP/DLG7 antibody BL3695 (Cat. No. A300-853A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HURP/DLG7 was also immunoprecipitated by rabbit anti-HURP/DLG7 antibodies BL3692 (Cat. No. A300-851A) and BL3693 (Cat. No. A300-852A), which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).