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Rabbit anti-HURP/DLG7 Antibody, Affinity Purified

Catalogue number:
A300-852A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to HURP/DLG7 immobilized on solid support. The epitope recognized by A300-852A maps to a region between residue 200 and 250 of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

DAP-5, Discs large homolog 7, discs large homolog associated protein 5, discs, large homolog 7, disks large-associated protein 5, disks large-associated protein DLG7, DLG7, hepatoma up-regulated protein, HURP

Species Reactivity:

Human

Immunogen:

between 200 and 250

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human HURP/DLG7 by western

Detection of human HURP/DLG7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HURP/DLG7 antibody A300-852A (lot A300-852A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HURP/DLG7 by western

Detection of human HURP/DLG7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HURP/DLG7 antibody A300-852A (lot A300-852A-2) used for IP at 6 µg per reaction. HURP/DLG7 was also immunoprecipitated by a previous lot of this antibody (A300-852A-1) and rabbit anti-HURP/DLG7 antibody A300-853A. For blotting immunoprecipitated HURP/DLG7, A300-852A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HURP/DLG7 by immunohi

Detection of human HURP/DLG7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HURP/DLG7 (Cat. No. A300-852A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB