Antibody was affinity purified using an epitope specific to HURP/DLG7 immobilized on solid support. The epitope recognized by A300-851A maps to a region between residue 150 and 200 of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DAP-5, Discs large homolog 7, discs large homolog associated protein 5, discs, large homolog 7, disks large-associated protein 5, disks large-associated protein DLG7, DLG7, hepatoma up-regulated protein, HURP
between 150 and 200
2 - 8°C
Detection of human HURP/DLG7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HURP/DLG7 antibody BL3692 (Cat. No. A300-851A) used for IP at 3 µg/mg lysate. HURP/DLG7 was also immunoprecipitated by rabbit anti-HURP/DLG7 antibodies BL3693 (Cat. No. A300-852A) and BL3695 (Cat. No. A300-853A), which recognize other epitopes. For blotting immunoprecipitated HURP/DLG7, BL3693 (A) and BL3695 (B) were used at 1 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).