Antibody was affinity purified using an epitope specific to PRMT5 immobilized on solid support. The epitope recognized by A300-849A maps to a region between residue 50 and 100 of Protein Arginine Methyltransferase 5 using the numbering given in entry NP_006100.2 (GeneID 10419). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
72 kDa ICln-binding protein, histone-arginine N-methyltransferase PRMT5, HMT1 hnRNP methyltransferase-like 5, HRMT1L5, IBP72, jak-binding protein 1, JBP1, protein arginine N-methyltransferase 5, shk1 kinase-binding protein 1 homolog, SKB1, SKB1 homolog, S
between 50 and 100
2 - 8°C
Detection of human PRMT5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRMT5 antibody A300-849A (lot A300-849A-2) used for IP at 6 µg per reaction. PRMT5 was also immunoprecipitated by a rabbit anti-PRMT5 antibodies A300-850A and BL3690. For blotting immunoprecipitated PRMT5, A300-849A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.