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Rabbit anti-LEDGF/p75 Antibody, Affinity Purified

Catalogue number:
A300-847A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to LEDGF/p75 immobilized on solid support. The epitope recognized by A300-847A maps to a region between residue 225 and 275 of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:

Antibodies

Alternative Names:

CLL-associated antigen KW-7, dense fine speckles 70 kDa protein, DFS 70, DFS70, LEDGF, lens epithelium-derived growth factor, p52, p75, PAIP, PC4 and SFRS1-interacting protein, PSIP2, transcriptional coactivator p52/p75, Transcriptional coactivator p75/p5

Species Reactivity:

Human, Mouse

Immunogen:

between 225 and 275

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human LEDGF/p75 by western

Detection of human LEDGF/p75 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody A300-847A (lot A300-847A-3) used for IP at 3 µg per reaction. LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody A300-848A. For blotting immunoprecipitated LEDGF-p75, A300-847A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human LEDGF/p75 by immunohi

Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (A300-847A lot 3) used at 1:5000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).