Antibody was affinity purified using an epitope specific to PPP4R3 Alpha immobilized on solid support. The epitope recognized by A300-839A maps to a region between residue 600 and 650 of human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) using the numbering given in entry NP_115949.3 (GeneID 55671). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FLFL1, KIAA2010, MSTP033, PP4R3, PP4R3A, serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK homolog 1, suppressor of mek1, SMEK1, smk1, smk-1
between 600 and 650
2 - 8°C
Detection of human PPP4R3 Alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R3 alpha antibody BL3120 (Cat. No. A300-839A) used for IP at 3 µg/mg lysate. PPP4R3 alpha was also immunoprecipitated by rabbit anti-PPP4R3 alpha antibody BL3121 (Cat. No. A300-840A), which recognizes a downstream epitope. For blotting immunoprecipitated PPP4R3 alpha, BL3121 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 0.5 seconds.