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Rabbit anti-PPP4R2 Antibody, Affinity Purified - A300-838A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PPP4R2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-838A
Name:
Rabbit anti-PPP4R2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PPP4R2 immobilized on solid support.The epitope recognized by A300-838A maps to a region between residue 370 and the C-terminus (residue 417) of human Protein Phosphatase 4, Regulatory Subunit 2 using the numbering given in entry NP_777567.1 (GeneID 151987).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
PP4R2, serine/threonine-protein phosphatase 4 regulatory subunit 2
Species Reactivity:
Human, Mouse
Immunogen:
Between 370 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PPP4R2 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody A300-838A (lot A300-838A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody A300-838A (lot A300-838A-2) used for IP at 6 µg per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (lot A300-838A-1). For blotting immunoprecipitated PPP4R2 , A300-838A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PPP4R2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PPP4R2 (Cat. No. A300-838A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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