Antibody was affinity purified using an epitope specific to PPP4R2 immobilized on solid support. The epitope recognized by A300-838A maps to a region between residue 370 and the C-terminus (residue 417) of human Protein Phosphatase 4, Regulatory Subunit 2 using the numbering given in entry NP_777567.1 (GeneID 151987). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP4R2, serine/threonine-protein phosphatase 4 regulatory subunit 2
between 370 and C-term
2 - 8°C
Detection of human and mouse PPP4R2 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody A300-838A (lot A300-838A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody A300-838A (lot A300-838A-2) used for IP at 6 µg per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (lot A300-838A-1). For blotting immunoprecipitated PPP4R2 , A300-838A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PPP4R2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PPP4R2 (Cat. No. A300-838A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB