Antibody was affinity purified using an epitope specific to PPP4C immobilized on solid support. The epitope recognized by A300-837A maps to a region between residue 880 and the C-terminus (residue 933) of human Protein Phosphatase 4, Regulatory Subunit 1 using the numbering given in entry NP_005125.1 (GeneID 9989). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MEG1, PP4(Rmeg), PP4R1, serine/threonine phosphatase 4, serine/threonine-protein phosphatase 4 regulatory subunit 1
between 880 and C-term
2 - 8°C
Detection of human PPP4R1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R1 antibody BL3114 (Cat. No. A300-837A) used for IP at 3 µg/mg lysate. PPP4R1 was also immunoprecipitated by rabbit anti-PPP4R1 antibody BL3111 (Cat. No. A300-836A), which recognizes an upstream epitope. For blotting immunoprecipitated PPP4R1, BL3111 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.