Antibody was affinity purified using an epitope specific to PPP4C immobilized on solid support. The epitope recognized by A300-836A maps to a region between residue 1 and 50 of human Protein Phosphatase 4, Regulatory Subunit 1 using the numbering given in entry NP_005125.1 (GeneID 9989). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MEG1, PP4(Rmeg), PP4R1, serine/threonine phosphatase 4, serine/threonine-protein phosphatase 4 regulatory subunit 1
between 1 and 50
2 - 8°C
Detection of human and mouse PPP4R1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP4R1 antibody BL3111 (Cat. No. A300-836A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPP4R1 was also immunoprecipitated by rabbit anti-PPP4R1 antibody BL3114 (Cat. No. A300-837A), which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).