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Rabbit anti-PPP4C Antibody, Affinity Purified - A300-835A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PPP4C Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-835A
Name:
Rabbit anti-PPP4C Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PPP4C immobilized on solid support.The epitope recognized by A300-835A maps to a region between residue 1 and 50 of human Protein Phosphatase 4, Catalytic Subunit using the numbering given in entry NP_002711.1 (GeneID 5531).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
epididymis secretory sperm binding protein, PP4, PP4C, PPH3, PPP4, PPX, PP-X, protein phosphatase 4 (formerly X), catalytic subunit, Protein phosphatase X, protein phosphatase X, catalytic subunit, serine/threonine-protein phosphatase 4 catalytic subunit
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PPP4C by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody A300-835A (lot A300-835A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PPP4C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP4C antibody A300-835A (lot A300-835A-3) used for IP at 6 µg per reaction. PPP4C was also immunoprecipitated by a previous lot of this antibody A300-835A-2. For blotting immunoprecipitated PPP4C, A300-835A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PPP4C by immunohistochemistry. Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PPP4C (Cat. No. A300-835A Lot3) used at a dilution of 1:1,000 (0.2 µg/ml). Detection: DAB

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Detection of mouse PPP4C by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PPP4C (Cat. No. A300-835A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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