Antibody was affinity purified using an epitope specific to DTX3L/BBAP immobilized on solid support. The epitope recognized by A300-834A maps to a region between residue 700 and the C-terminus (residue 740) of human Deltex 3-Like Protein (B-Lymphoma- and BAL-Associated Protein) using the numbering given in entry NP_612144.1 (GeneID 151636). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like, E3 ubiquitin ligase, deltex 3-like, E3 ubiquitin-protein ligase DTX3L, Protein deltex-3-like, rhysin2, rhysin-2, RING-type E3 ubiquitin transferase DTX3L, RNF143
between 700 and C-term
2 - 8°C
Detection of human and mouse DTX3L/BBAP by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DTX3L/BBAP antibody BL3624 (Cat. No. A300-834A) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). DTX3L/BBAP was also immunoprecipitated by rabbit anti-DTX3L/BBAP antibodies BL3622 (Cat. No. A300-833A) and BL3623, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).