Antibody was affinity purified using an epitope specific to E4F1 immobilized on solid support. The epitope recognized by A300-832A maps to a region between residue 725 and the C-terminus (residue 784) of human E4F1 (p120E4F) using the numbering given in entry NP_004415.1 (GeneID 1877). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E4F, E4F transcription factor 1, p120E4F, p50E4F, putative E3 ubiquitin-protein ligase E4F1, RING-type E3 ubiquitin transferase E4F1, transcription factor E4F, transcription factor E4F1
between 725 and C-term
2 - 8°C
Detection of human E4F1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-E4F1 antibody A300-832A (lot A300-832A-3) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. E4F1 was also immunoprecipitated by a previous lot (A300-832A-2) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).