Antibody was affinity purified using an epitope specific to E4F1 immobilized on solid support. The epitope recognized by A300-831A maps to a region between residue 275 and 325 of human E4F1 (p120E4F) using the numbering given in entry NP_004415.1 (GeneID 1877). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
E4F, E4F transcription factor 1, p120E4F, p50E4F, putative E3 ubiquitin-protein ligase E4F1, RING-type E3 ubiquitin transferase E4F1, transcription factor E4F, transcription factor E4F1
between 275 and 325
2 - 8°C
Detection of human E4F1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (H; 50 µg), HEK293T (T; 50 µg), MCF-7 (M; 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50µg) and NIH 3T3 (3; 50 µg) cells. Antibodies: Affinity purified rabbit anti-E4F1 antibody A300-831A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). E4F1 was also immunoprecipitated by rabbit anti-E4F1 antibody A300-832A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).