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Rabbit anti-USP4 Antibody, Affinity Purified

Catalogue number:
A300-830A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to USP4 immobilized on solid support. The epitope recognized by A300-830A maps to a region between residue 925 and the C-terminus (residue 963) of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

deubiquitinating enzyme 4, ubiquitin carboxyl-terminal esterase 4, ubiquitin carboxyl-terminal hydrolase 4, ubiquitin specific peptidase 4 (proto-oncogene), ubiquitin specific protease 4 (proto-oncogene), ubiquitin thioesterase 4, ubiquitin thiolesterase

Species Reactivity:

Human

Immunogen:

between 925 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse USP4 by wes

Detection of human and mouse USP4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody A300-830A (lot A300-830A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human USP4 by western blot

Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody A300-830A (lot A300-830A-2) used for IP at 6 µg per reaction. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody A300-829A. For blotting immunoprecipitated USP4, A300-830A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.