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Rabbit anti-TPR Antibody, Affinity Purified

Catalogue number:
A300-825A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to TPR immobilized on solid support. The epitope recognized by A300-825A maps to a region between residue 600 and 650 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human TPR by western blot a
Product Type:

Antibodies

Alternative Names:

megator, NPC-associated intranuclear protein, nuclear pore complex-associated protein TPR, nucleoprotein TPR, translocated promoter region (to activated MET oncogene), translocated promoter region protein, tumor potentiating region

Species Reactivity:

Human

Immunogen:

between 600 and 650

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TPR by western blot a

Detection of human TPR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TPR antibody BL2148 (Cat. No. A300-825A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TPR was also immunoprecipitated by rabbit anti-TPR antibodies BL2149 (Cat. No. A300-826A), BL2150 (Cat. No. A300-827A) and BL2151 (Cat. No. A300-828A), which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).