Rabbit anti-Histone H3 Antibody, Affinity Purified - A300-823A - 20 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Histone H3 immobilized on solid support. The epitope recognized by A300-823A maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC, IHC-IF
Detection of human Histone H3 by western blot. Samples: Histones (15, 7.5, 3.7, 1.8, 0.9 µg) isolated from HeLa cells. Antibody: Affinity purified rabbit anti-Histone H3 antibody A300-823A (lot A300-823A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Histone H3 by western blot of immunoprecipitates. Samples: Histones (1.0 mg per IP reaction; 20% of IP loaded) isolated from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Histone H3 antibody A300-823A (lot A300-823A-3) used for IP at 3 µg per reaction. Histone H3 was also immunoprecipitated by a previous lot of this antibody (lot A300-823A-2) and rabbit anti-Histone H3 antibody A300-822A. For blotting immunoprecipitated Histone H3, A300-823A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human Histone H3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 (Cat. No. A300-832A Lot3) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB
Detection of human Histone H3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 (Cat. No. A300-832A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-201D4) used at a dilution of 1:100
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