Antibody was affinity purified using an epitope specific to Histone H3 immobilized on solid support. The epitope recognized by A300-823A maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
H3 histone family, member A, H3/A, H3FA, histone 1, H3a, histone cluster 1, H3a, histone H3.1, histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
between 100 and C-term
2 - 8°C
Detection of human Histone H3 by western blot of immunoprecipitates. Samples: Histones (1.0 mg per IP reaction; 20% of IP loaded) isolated from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Histone H3 antibody A300-823A (lot A300-823A-3) used for IP at 3 µg per reaction. Histone H3 was also immunoprecipitated by a previous lot of this antibody (lot A300-823A-2) and rabbit anti-Histone H3 antibody A300-822A. For blotting immunoprecipitated Histone H3, A300-823A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human Histone H3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 (Cat. No. A300-832A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-201D4) used at a dilution of 1:100