Rabbit anti-Histone H3 Antibody, Affinity Purified - A300-822A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Histone H3 immobilized on solid support. The epitope recognized by A300-822A maps to a region between residue 1 and 50 of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP
Detection of human Histone H3 by western blot of immunoprecipitates. Samples: Histones (1 mg for IP, 20% of IP loaded) isolated from HEK293T cells. Antibodies: Affinity purified rabbit anti-Histone H3 antibody BL3588 (Cat. No. A300-822A) used for IP at 3 µg/mg histones. Histone H3 was also immunoprecipitated by rabbit anti-Histone H3 antibody BL3591 (Cat. No. A300-823A), which recognizes a downstream epitope. For blotting immunoprecipitated Histone H3, BL3591 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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