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Rabbit anti-RBM15 Antibody, Affinity Purified

Catalogue number:
A300-821A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to RBM15 immobilized on solid support. The epitope recognized by A300-821A maps to a region between residue 550 and 600 of human RNA Binding Motif Protein 15 using the numbering given in entry NP_073605.4 (GeneID 64783). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

one twenty two protein, one twenty-two, one-twenty two protein 1, OTT, OTT1, putative RNA-binding protein 15, RNA-binding motif protein 15, RNA-binding protein 15, SPEN

Species Reactivity:

Human

Immunogen:

between 550 and 600

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RBM15 by we

Detection of human and mouse RBM15 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM15 antibody A300-821A (lot A300-821A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RBM15 by western blot

Detection of human RBM15 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM15 antibody A300-821A (lot A300-821A-2) used for IP at 6 µg per reaction. RBM15 was also immunoprecipitated by rabbit anti-RBM15 antibody A300-821A-1 For blotting immunoprecipitated RBM15, A300-821A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.