Antibody was affinity purified using an epitope specific to ASPP2 immobilized on solid support. The epitope recognized by A300-820A maps to a region between residue 825 and 875 of human Apoptosis-Stimulating Protein of p53, 2 (53BP2) using the numbering given in entry NP_001026855.1 (GeneID 7159). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
53BP2, apoptosis-stimulating of p53 protein 2, apoptosis-stimulating protein of p53, 2, ASPP2, BBP, BCL2-binding protein, p53-binding protein 2, P53BP2, PPP1R13A, renal carcinoma antigen NY-REN-51, tumor suppressor p53-binding protein 2
between 825 and 875
2 - 8°C
Detection of human ASPP2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ASPP2 antibody BL3602 (Cat. No. A300-820A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). ASPP2 was also immunoprecipitated by rabbit anti-ASPP2 antibody BL3600 (Cat. No. A300-819A), which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human and mouse ASPP2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-ASPP2 (Cat. No. A300-820A Lot1) used at a dilution of 1:5,000 (0.2µg/ml) and 1:1,000 (1µg/ml). Detection: DAB