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Rabbit anti-ASPP2 Antibody, Affinity Purified

Catalogue number:
A300-819A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to ASPP2 immobilized on solid support. The epitope recognized by A300-819A maps to a region between residue 425 and 475 of human Apoptosis-Stimulating Protein of p53, 2 (53BP2) using the numbering given in entry NP_001026855.1 (GeneID 7159). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

53BP2, apoptosis-stimulating of p53 protein 2, apoptosis-stimulating protein of p53, 2, ASPP2, BBP, BCL2-binding protein, p53-binding protein 2, P53BP2, PPP1R13A, renal carcinoma antigen NY-REN-51, tumor suppressor p53-binding protein 2

Species Reactivity:

Human

Immunogen:

between 425 and 475

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human ASPP2 by western blot

Detection of human ASPP2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ASPP2 antibody BL3600 (Cat. No. A300-819A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). ASPP2 was also immunoprecipitated by rabbit anti-ASPP2 antibody BL3602 (Cat. No. A300-820A), which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).