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Rabbit anti-CoCoA Antibody, Affinity Purified

Catalogue number:
A300-818A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to CoCoA immobilized on solid support. The epitope recognized by A300-818A maps to a region between residue 650 and the C-terminus (residue 691) of human Coiled-Coil Transcriptional Coactivator 1 using the numbering given in entry NP_065949.1 (GeneID 57658). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human and mouse CoCoA by WB
Product Type:

Antibodies

Alternative Names:

calcium-binding and coiled-coil domain-containing protein 1, calphoglin, Cocoa, coiled-coil coactivator protein, coiled-coil leucine zipper coactivator 1, inorganic pyrophosphatase activator, PP13275, sarcoma antigen NY-SAR-3

Species Reactivity:

Human, Mouse

Immunogen:

between 650 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse CoCoA by WB

Detection of human and mouse CoCoA by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 µg), HEK293T (T; 50 µg), NIH 3T3 (N; 50 µg), Ramos (R; 50µg), and MCF-7 (M; 50 µg for WB; 1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-CoCoA antibody BL2456 (Cat. No. A300-818A) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). CoCoA was also immunoprecipitated by rabbit anti-CoCoA antibody BL2455 (Cat. No. A300-817A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).