Antibody was affinity purified using an epitope specific to CoCoA immobilized on solid support. The epitope recognized by A300-817A maps to a region between residue 1 and 50 of human Coiled-Coil Transcriptional Coactivator 1 using the numbering given in entry NP_065949.1 (GeneID 57658). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
calcium-binding and coiled-coil domain-containing protein 1, calphoglin, Cocoa, coiled-coil coactivator protein, coiled-coil leucine zipper coactivator 1, inorganic pyrophosphatase activator, PP13275, sarcoma antigen NY-SAR-3
between 1 and 50
2 - 8°C
Detection of human CoCoA by western blot of immunoprecipitates. Samples: Whole cell lysate (50 µg for WB; 1 mg for IP, 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-CoCoA antibody BL2455 (Cat. No. A300-817A) used for IP at 3 µg/mg lysate (B). CoCoA was also immunoprecipitated by rabbit anti-CoCoA antibody BL2456 (Cat. No. A300-818A), which recognizes a downstream epitope. For blotting immunoprecipitated CoCoA, BL2456 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.