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Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified

Catalogue number:
A300-816A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to TIF1 Alpha/TRIM24 immobilized on solid support. The epitope recognized by A300-816A maps to a region between residue 1,000 and the C-terminus (residue 1050) of human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24) using the numbering given in entry NP_056989.2 (GeneID 8805). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

E3 ubiquitin-protein ligase TRIM24, hTIF1, PTC6, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, TIF1-alpha, transcription intermediary factor 1-alpha, transcriptional intermediary factor 1, Trip

Species Reactivity:

Human

Immunogen:

between 1000 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TIF1 alpha/TRIM24 by

Detection of human TIF1 alpha/TRIM24 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TIF1 alpha/TRIM24 antibody A300-816A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody A300-815A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).