Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified

Name: Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_00071096
Price: 436 GBP
Availability: InStock

Catalogue number:

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A300-815A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl (200 µg/ml) _$$_

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Currency:
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£436.00

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Estimated delivery Wed 1 May
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Applications:

WB, IP, IHC, ChIP-Seq

Antibody Host:

Rabbit

Target:

TIF1 Alpha/TRIM24

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 700 and 750

Alternative Names:

TF1A; TIF1; Tripartite motif-containing protein 24; transcriptional intermediary factor 1; transcription intermediary factor 1-alpha; TIF1-alpha; TIF1ALPHA; E3 ubiquitin-protein ligase TRIM24; RNF82; RING-type E3 ubiquitin transferase TIF1-alpha; RING fin

Product Description:

Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse TIF1 Alpha/TRIM24 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody A300-815A (lot A300-815A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TIF1 Alpha/TRIM24 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody A300-815A (lot A300-815A-2) used for IP at 6 µg per reaction. TIF1 Alpha/TRIM24 was also immunoprecipitated by a previous lot of this antibody (lot A300-815A-1) and rabbit anti-TIF1 Alpha/TRIM24 antibody A300-816A For blotting immunoprecipitated TIF1 Alpha/TRIM24, A300-815A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 (Cat. No. A300-815A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody A300-815A and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody A300-815A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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