Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:
Over 100,000 Antibodies Performance Guaranteed
Find Your Antibody Quickly & Easily
T-Cell Marker Antibodies

Receive High Quality Leukaemia & Lymphoma Biospeciemens Within 24 Hours

Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified - A300-815A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified
print page
Size: 20 µg
£302.00
GBP | Euro | USD
Add to basket
Catalogue number:
A300-815A
Name:
Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified
Supplier:
Availability:

Antibody was affinity purified using an epitope specific to TIF1 Alpha/TRIM24 immobilized on solid support.The epitope recognized by A300-815A maps to a region between residue 700 and 750 of human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24 ) using the numbering given in entry NP_056989.2 (GeneID 8805).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, ChIP-Seq

Product Type:
Antibodies
Alternative Names:
E3 ubiquitin-protein ligase TRIM24, hTIF1, PTC6, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, TIF1-alpha, transcription intermediary factor 1-alpha, transcriptional intermediary factor 1, Trip
Species Reactivity:
Human, Mouse
Immunogen:
Between 700 and 750
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
thumbnail

Detection of human and mouse TIF1 Alpha/TRIM24 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody A300-815A (lot A300-815A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

thumbnail

Detection of human TIF1 Alpha/TRIM24 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody A300-815A (lot A300-815A-2) used for IP at 6 µg per reaction. TIF1 Alpha/TRIM24 was also immunoprecipitated by a previous lot of this antibody (lot A300-815A-1) and rabbit anti-TIF1 Alpha/TRIM24 antibody A300-816A For blotting immunoprecipitated TIF1 Alpha/TRIM24, A300-815A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

thumbnail

Detection of human TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TIF1 alpha/TRIM24 (Cat. No. A300-815A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

thumbnail

Detection of mouse TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 (Cat. No. A300-815A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

thumbnail

Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody A300-815A and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody A300-815A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

Contact Our Customer Service Team
HuaBio