Antibody was affinity purified using an epitope specific to SAFB1 immobilized on solid support. The epitope recognized by A300-812A maps to a region between residue 875 and the C-terminus (residue 915) of human Scaffold Attachment Factor B using the numbering given in entry NP_002958.2 (GeneID 6294). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
glutathione S-transferase fusion protein, HAP, heat-shock protein (HSP27) estrogen response element and TATA box-binding protein, HET, Hsp27 ERE-TATA binding protein, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein
between 875 and C-term
2 - 8°C
Detection of human and mouse SAFB1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SAFB1 antibody BL2446 (Cat. No. A300-812A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody BL2445 (Cat. No. A300-811A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).