Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support. The epitope recognized by A300-808A maps to a region between residue 675 and the C-terminus (residue 725) of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
CT139, kinesin-like protein 6, kinesin-like protein KIF2C, KNSL6, MCAK, mitotic centromere-associated kinesin, testis tissue sperm-binding protein Li 68n
between 675 and C-term
2 - 8°C
Detection of human MCAK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MCAK antibody BL3508 (Cat. No. A300-808A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). MCAK was also immunoprecipitated by rabbit anti-MCAK antibodies BL3505 (Cat. No. A300-806A) and BL3507 (Cat. No. A300-807A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).