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T-Cell Marker Antibodies

Antibodies for Immuno-Oncology Research

Achieve High Yield Mammalian Protein Production In Suspension HEK 293 & CHO Derived Cells

Rabbit anti-MCAK Antibody, Affinity Purified - A300-808A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MCAK Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-808A
Name:
Rabbit anti-MCAK Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support.The epitope recognized by A300-808A maps to a region between residue 675 and the C-terminus (residue 725) of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CT139, kinesin-like protein 6, kinesin-like protein KIF2C, KNSL6, MCAK, mitotic centromere-associated kinesin, testis tissue sperm-binding protein Li 68n
Species Reactivity:
Human
Immunogen:
Between 675 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MCAK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MCAK antibody BL3508 (Cat. No. A300-808A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). MCAK was also immunoprecipitated by rabbit anti-MCAK antibodies BL3505 (Cat. No. A300-806A) and BL3507 (Cat. No. A300-807A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

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