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Rabbit anti-MCAK Antibody, Affinity Purified

Catalogue number:
A300-806A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support. The epitope recognized by A300-806A maps to a region between residue 50 and 100 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:

Antibodies

Alternative Names:

CT139, kinesin-like protein 6, kinesin-like protein KIF2C, KNSL6, MCAK, mitotic centromere-associated kinesin, testis tissue sperm-binding protein Li 68n

Species Reactivity:

Human

Immunogen:

between 50 and 100

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human MCAK by western blot

Detection of human MCAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MCAK antibodies BL3505 (Cat. No. A300-806A), BL3507 (Cat. No. A300-807A), and BL3508 (Cat. No. A300-808A) used for IP at 3 µg/mg lysate. For blotting immunoprecipitated MCAK, BL3507 (left panel) and BL3508 (right panel) were used at 1 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (left) and 1 second (right).

Detection of human MCAK by immunohistoch

Detection of human MCAK by immunohistochemistry. Sample: FFPE section of human skin carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-MCAK (Cat. No. A300-806A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB