Antibody was affinity purified using an epitope specific to CASC5 immobilized on solid support. The epitope recognized by A300-804A maps to a region between residue 250 and 300 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2 (GeneID 57082). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AF15Q14, ALL1-fused gene from chromosome 15q14 protein, Blinkin, blinkin, bub-linking kinetochore protein, Bub-linking kinetochore protein, cancer susceptibility candidate 5, cancer susceptibility candidate gene 5 protein, cancer/testis antigen 29, CASC5,
between 250 and 300
2 - 8°C
Detection of human CASC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CASC5 antibody BL3462 (Cat. No. A300-804A) used for IP at 3 µg/mg lysate. CASC5 was also immunoprecipitated by rabbit anti-CASC5 antibody BL3463 (Cat. No. A300-805A). For blotting immunoprecipitated CASC5, BL3463 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.