Antibody was affinity purified using an epitope specific to SUPT6H immobilized on solid support. The epitope recognized by A300-803A maps to a region between residue 1553 and the C-terminus (residue 1583) of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
emb-5, histone chaperone suppressor of Ty6, hSPT6, SPT6, SPT6H, suppressor of Ty 6 homolog, tat-cotransactivator 2 protein, tat-CT2 protein, transcription elongation factor SPT6
between 1553 and C-term
2 - 8°C
Detection of human SUPT6H by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies BL3467 (Cat. No. A300-801A), BL3468 (Cat. No. A300-802A), and BL3470 (Cat. No. A300-803A) used for IP at 3 µg/mg lysate (A and B). For blotting immunoprecipitated SUPT6H BL3467 (left panel) or BL3468 (right panel) was used at 0.04 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).