Antibody was affinity purified using an epitope specific to SUPT6H immobilized on solid support. The epitope recognized by A300-802A maps to a region between residue 1050 and 1100 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
emb-5, histone chaperone suppressor of Ty6, hSPT6, SPT6, SPT6H, suppressor of Ty 6 homolog, tat-cotransactivator 2 protein, tat-CT2 protein, transcription elongation factor SPT6
between 1050 and 1100
2 - 8°C
Detection of human SUPT6H by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUPT6H antibody A300-802A (lot A300-802A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.