Antibody was affinity purified using an epitope specific to SUPT6H immobilized on solid support. The epitope recognized by A300-801A maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
emb-5, histone chaperone suppressor of Ty6, hSPT6, SPT6, SPT6H, suppressor of Ty 6 homolog, tat-cotransactivator 2 protein, tat-CT2 protein, transcription elongation factor SPT6
between 425 and 475
2 - 8°C
Detection of human SUPT6H by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibody BL3467 (Cat. No. A300-801A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SUPT6H was also immunoprecipitated by rabbit anti-SUPT6H antibody BL3470 (Cat. No. A300-803A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human and mouse SUPT6H by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H (Cat. No. A300-801A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB