Antibody was affinity purified using an epitope specific to DHX33 immobilized on solid support. The epitope recognized by A300-799A maps to a region between residue 1 and 50 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 33 using the numbering given in entry NP_064547.2 (GeneID 56919). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DHX33, DDX33, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33, DEAH (Asp-Glu-Ala-His) box polypeptide 33, DEAH box protein 33, putative ATP-dependent RNA helicase DHX33
between 1 and 50
2 - 8°C
Detection of human DHX33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX33 antibody A300-799A (lot A300-799A-2) used for IP at 6 µg per reaction. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody A300-800A For blotting immunoprecipitated DHX33, A300-799A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human DHX33 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 (Cat. No. A300-799A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).