Antibody was affinity purified using an epitope specific to NIF1 immobilized on solid support. The epitope recognized by A300-798A maps to a region between residue 1300 and the C-terminus (residue 1342) of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MCPH10, NIF1, NIF-1, NIF2, NRC-interacting factor 1, zinc finger protein 335, zinc-finger/leucine-zipper co-transducer NIF1
between 1300 and C-term
2 - 8°C
Detection of human and mouse NIF1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody BL2739 (Cat. No. A300-798A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies BL2737 (Cat. No. A300-797A) and BL2738. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human NIF1 by immunohistochemistry. Sample: FFPE section of human Hodgkin's lymphoma. Antibody: Affinity purified rabbit anti- NIF1 (Cat. No. A300-798A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB