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Rabbit anti-NIF1 Antibody, Affinity Purified

Catalogue number:
A300-797A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to NIF1 immobilized on solid support. The epitope recognized by A300-797A maps to a region between residue 300 and 350 of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:

Antibodies

Alternative Names:

MCPH10, NIF1, NIF-1, NIF2, NRC-interacting factor 1, zinc finger protein 335, zinc-finger/leucine-zipper co-transducer NIF1

Species Reactivity:

Human, Mouse

Immunogen:

between 300 and 350

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human NIF1 by western blot

Detection of human NIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody BL2737 (Cat. No. A300-797A) used IP at 3 µg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies BL2738 and BL2739 (Cat. No. A300-798A). For blotting immunoprecipitated NIF1, BL2739 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human and mouse NIF1 by imm

Detection of human and mouse NIF1 by immunohistochemistry. Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 (Cat. No. A300-797A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB