Antibody was affinity purified using an epitope specific to NIF1 immobilized on solid support. The epitope recognized by A300-797A maps to a region between residue 300 and 350 of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MCPH10, NIF1, NIF-1, NIF2, NRC-interacting factor 1, zinc finger protein 335, zinc-finger/leucine-zipper co-transducer NIF1
between 300 and 350
2 - 8°C
Detection of human NIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody BL2737 (Cat. No. A300-797A) used IP at 3 µg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies BL2738 and BL2739 (Cat. No. A300-798A). For blotting immunoprecipitated NIF1, BL2739 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.