Antibody was affinity purified using an epitope specific to DC8 immobilized on solid support. The epitope recognized by A300-795A maps to a region between residue 230 and the C-terminus (residue 281) of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C1orf48, DC8, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog
between 230 and C-term
2 - 8°C
Detection of human DC8 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DC8 antibody A300-795A (lot A300-795A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti- DC8 antibody A300-795A (lot A300-795A-2) used for IP at 6 µg per reaction. DC8 was also immunoprecipitated by rabbit anti-DC8 antibody A300-794A. For blotting immunoprecipitated DC8, A300-795A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.