www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-DC8 Antibody, Affinity Purified

Catalogue number:
A300-795A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to DC8 immobilized on solid support. The epitope recognized by A300-795A maps to a region between residue 230 and the C-terminus (residue 281) of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

C1orf48, DC8, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog

Species Reactivity:

Human

Immunogen:

between 230 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DC8 by western blot.

Detection of human DC8 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DC8 antibody A300-795A (lot A300-795A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DC8 by western blot o

Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti- DC8 antibody A300-795A (lot A300-795A-2) used for IP at 6 µg per reaction. DC8 was also immunoprecipitated by rabbit anti-DC8 antibody A300-794A. For blotting immunoprecipitated DC8, A300-795A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.