Antibody was affinity purified using an epitope specific to DC8 immobilized on solid support. The epitope recognized by A300-794A maps to a region between residue 150 and 200 of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C1orf48, DC8, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog
between 150 and 200
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti- DC8 antibody A300-794A (lot A300-794A-2) used for IP at 6 µg per reaction. DC8 was also immunoprecipitated by rabbit anti-DC8 antibody A300-795A. For blotting immunoprecipitated DC8, A300-795A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.