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Rabbit anti-NCOA5/CIA Antibody, Affinity Purified - A300-790A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-NCOA5/CIA Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-790A
Name:
Rabbit anti-NCOA5/CIA Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to NCOA5/CIA immobilized on solid support.The epitope recognized by A300-790A maps to a region between residue 525 and the C-terminus (residue 579) of human Nuclear Receptor Coactivator 5 (Coactivator Independent of AF-2) using the numbering given in entry NP_066018.1 (GeneID 57727).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
bA465L10.6, CIA, coactivator independent of AF-2, NCoA-5, nuclear receptor coactivator 5
Species Reactivity:
Human, Mouse
Immunogen:
Between 525 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse NCOA5 (CIA) by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NCOA5 (CIA) antibody BL2432 (Cat. No. A300-790A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). NCOA5 (CIA) was also immunoprecipitated by rabbit anti-NCOA5 (CIA) antibody BL2431 (Cat. No. A300-789A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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Detection of human NCOA5 (CIA) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NCOA5 (CIA) (Cat. No. A300-790A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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