Antibody was affinity purified using an epitope specific to NCOA5/CIA immobilized on solid support. The epitope recognized by A300-790A maps to a region between residue 525 and the C-terminus (residue 579) of human Nuclear Receptor Coactivator 5 (Coactivator Independent of AF-2) using the numbering given in entry NP_066018.1 (GeneID 57727). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bA465L10.6, CIA, coactivator independent of AF-2, NCoA-5, nuclear receptor coactivator 5
between 525 and C-term
2 - 8°C
Detection of human and mouse NCOA5 (CIA) by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NCOA5 (CIA) antibody BL2432 (Cat. No. A300-790A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). NCOA5 (CIA) was also immunoprecipitated by rabbit anti-NCOA5 (CIA) antibody BL2431 (Cat. No. A300-789A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).