Antibody was affinity purified using an epitope specific to CDT1 immobilized on solid support. The epitope recognized by A300-786A maps to a region between residue 500 and the C-terminus (residue 546) of human Chromatin Licensing and DNA Replication Factor 1 using the numbering given in entry NP_112190.1 (GeneID 81620). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA replication factor Cdt1, Double parked homolog, Double parked, Drosophila, homolog of, DUP, RIS2
between 500 and C-term
2 - 8°C
Detection of human CDT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CDT1 antibody BL2396 (Cat. No. A300-786A) used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A and B).