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Rabbit anti-NCOA62 Antibody, Affinity Purified - A300-785A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-NCOA62 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-785A
Name:
Rabbit anti-NCOA62 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to NCOA62 immobilized on solid support.The epitope recognized by A300-785A maps to a region between residue 500 and the C-terminus (residue 536) of human Nuclear Receptor Coactivator, 62 kD (SKIIP, SKI Interacting Protein) using the numbering given in entry NP_036377.1 (GeneID 22938).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
Bx42, FUN20, homolog of Drosophila BX42, NCOA-62, nuclear protein SkiP, nuclear receptor coactivator NCoA-62, nuclear receptor coactivator, 62-kD, Prp45, PRPF45, SKI interacting protein, ski-interacting protein, SKIIP, SKIP, SKIP1, SNW domain-containing p
Species Reactivity:
Human, Mouse
Immunogen:
Between 500 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse NCOA62 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NCOA62 antibody BL2751 (Cat. No. A300-785A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). NCOA62 was also immunoprecipitated by rabbit anti-NCOA62 antibody BL2750 (Cat. No. A300-784A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

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Detection of human and mouse NCOA62 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NCOA62 (Cat. No. A300-785A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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