Antibody was affinity purified using an epitope specific to ZWINT-1 immobilized on solid support. The epitope recognized by A300-781A maps to a region between residue 225 and the C-terminus (residue 277) of human ZW10 Interactor-1 using the numbering given in entry NP_008988.2 (GeneID 11130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
human ZW10 interacting protein-1, HZwint-1, KNTC2AP, SIP30, SNAP25 interacting protein of 30 kDa, ZW10 interactor, ZW10 interactor, kinetochore protein, ZW10-interacting protein 1, ZWINT1, zwint-1
between 225 and C-term
2 - 8°C
Detection of human ZWINT-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. A300-781A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. A300-780A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human ZWINT-1 by immunocytochemistry. Sample: formaldehyde-fixed HeLa cells. Antibody: Affinity purified rabbit anti- ZWINT-1 ( Cat. No. A300-781A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. A120-501F) used at a dilution of 1:100 (right).