Antibody was affinity purified using an epitope specific to ZWINT-1 immobilized on solid support. The epitope recognized by A300-780A maps to a region between residue 1 and 50 of human ZW10 Interactor-1 using the numbering given in entry NP_008988.2 (GeneID 11130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
human ZW10 interacting protein-1, HZwint-1, KNTC2AP, SIP30, SNAP25 interacting protein of 30 kDa, ZW10 interactor, ZW10 interactor, kinetochore protein, ZW10-interacting protein 1, ZWINT1, zwint-1
between 1 and 50
2 - 8°C
Detection of human ZWINT-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. A300-780A) used for IP at 3 µg/mg lysate. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. A300-781A). For blotting immunoprecipitated ZWINT-1, BL3453 was used at 1 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.