Antibody was affinity purified using an epitope specific to MED18 immobilized on solid support. The epitope recognized by A300-777A maps to a region between residue 150 and the C-terminus (residue 208) of human Mediator of RNA Polymerase II Transcription, subunit 18 using the numbering given in entry NP_060108.1 (GeneID 54797). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Mediator complex subunit 18, mediator of RNA polymerase II transcription subunit 18, mediator of RNA polymerase II transcription, subunit 18 homolog, p28b, SRB5, TRAP/mediator complex subunit p28b
between 150 and C-term
2 - 8°C
Detection of human and mouse MED18 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MED18 antibody BL2854 (Cat. No. A300-777A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).