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Rabbit anti-MED18 Antibody, Affinity Purified

Catalogue number:
20 µg
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Antibody was affinity purified using an epitope specific to MED18 immobilized on solid support. The epitope recognized by A300-777A maps to a region between residue 150 and the C-terminus (residue 208) of human Mediator of RNA Polymerase II Transcription, subunit 18 using the numbering given in entry NP_060108.1 (GeneID 54797). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:


Alternative Names:

Mediator complex subunit 18, mediator of RNA polymerase II transcription subunit 18, mediator of RNA polymerase II transcription, subunit 18 homolog, p28b, SRB5, TRAP/mediator complex subunit p28b

Species Reactivity:

Human, Mouse


between 150 and C-term

Storage Temp:

2 - 8°C

Antibody Host:


Antibody Isotype:

Whole IgG

Antibody Type:




Detection of human and mouse MED18 by WB

Detection of human and mouse MED18 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MED18 antibody BL2854 (Cat. No. A300-777A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human MED18 by immunohistoc

Detection of human MED18 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MED18 (Cat. No. A300-777A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB