Antibody was affinity purified using an epitope specific to MIS12 immobilized on solid support. The epitope recognized by A300-775A maps to a region between residue 1 and 50 of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
2510025F08Rik, hMis12, homolog of yeast Mis12, KNTC2AP, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog, MTW1, protein MIS12 homolog
between 1 and 50
2 - 8°C
Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody A300-775A (lot A300-775A-2) used for IP at 3 µg per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody A300-776A. For blotting immunoprecipitated MIS12, A300-776A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.