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Rabbit anti-MIS12 Antibody, Affinity Purified

Catalogue number:
A300-775A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to MIS12 immobilized on solid support. The epitope recognized by A300-775A maps to a region between residue 1 and 50 of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

2510025F08Rik, hMis12, homolog of yeast Mis12, KNTC2AP, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog, MTW1, protein MIS12 homolog

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human MIS12 by western blot

Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody A300-775A (lot A300-775A-2) used for IP at 3 µg per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody A300-776A. For blotting immunoprecipitated MIS12, A300-776A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.